Hotspot

accD-psaI

LSC · 58108-59014 · 907 bp

Back to Baccharis

Pi 0.0379

MarkerSeek score 77.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 50
pi: 0.0379
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.4	58.0	1045-1111 bp	1.0	80.6
accD-psaI_p2	AAAGGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.4	58.0	1044-1110 bp	1.0	80.6
accD-psaI_p3	AAAAGGCGTTCTGAGTGAGT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.4	58.3	1035-1101 bp	1.0	78.7
accD-psaI_p4	AAAGGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.4	58.3	1034-1100 bp	1.0	78.7
accD-psaI_p5	AAAGGCGTTCTGAGTGAGTT	AGGCCTACTAAAGGGACCAA	45.0	50.0	57.4	57.6	1014-1080 bp	1.0	78.0