Hotspot

ndhC-trnV-UAC

LSC · 50213-50943 · 731 bp

Back to Baccharis

Pi 0.0235
MarkerSeek score 71.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
731
variable_sites
24
pi
0.0235
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCGGAGCTAAAAATCCCGAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 56.9 57.8 832-875 bp 1.0 76.0
ndhC-trnV-UAC_p2 TCGGAGCTAAAAATCCCGAAA AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.7 57.8 832-875 bp 1.0 73.8
ndhC-trnV-UAC_p3 TCGGAGCTAAAAATCCCGAA GAAGGTCTACGGTTCGAGTC 45.0 55.0 56.9 57.5 831-874 bp 1.0 73.2
ndhC-trnV-UAC_p4 TCGGAGCTAAAAATCCCGAA GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.9 58.7 820-863 bp 1.0 72.2
ndhC-trnV-UAC_p5 TCGGAGCTAAAAATCCCGAAA GAAGGTCTACGGTTCGAGTC 42.9 55.0 57.7 57.5 831-874 bp 1.0 71.0