Hotspot

trnG-UCC-trnT-GGU

LSC · 30622-30903 · 282 bp

Back to Baccharis

Pi 0.0402
MarkerSeek score 71.50
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
282
variable_sites
15
pi
0.0402
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 AATGAAACCGAAAGACCCCT TCCCCTATCGGATCTGAACC 45.0 55.0 57.0 58.0 425-659 bp 0.666667 60.3
trnG-UCC-trnT-GGU_p2 ATGAAACCGAAAGACCCCTT TCCCCTATCGGATCTGAACC 45.0 55.0 57.0 58.0 424-658 bp 0.666667 60.3
trnG-UCC-trnT-GGU_p3 AATGAAACCGAAAGACCCCT GATCTGAACCGAGGGCTTAC 45.0 55.0 57.0 57.8 415-649 bp 0.666667 58.6
trnG-UCC-trnT-GGU_p4 ATGAAACCGAAAGACCCCTT GATCTGAACCGAGGGCTTAC 45.0 55.0 57.0 57.8 414-648 bp 0.666667 58.6
trnG-UCC-trnT-GGU_p5 GAATGAAACCGAAAGACCCCT TCCCCTATCGGATCTGAACC 47.6 55.0 58.2 58.0 426-660 bp 0.666667 58.4