Hotspot

trnY-GUA-rpoB

LSC · 11451-12535 · 1085 bp

Back to Baccharis

Pi 0.0123
MarkerSeek score 55.40
Primer available yes
Label trnY-GUA-rpoB

Feature Metadata

label_name
trnY-GUA-rpoB
feature_type
igs
region
LSC
length_bp
1085
variable_sites
19
pi
0.0123
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-rpoB_p1 CAACGAATTTACAGTCCGCC TCTGATTAAATCCAGGTATTGTGG 50.0 37.5 57.5 56.5 1159-1363 bp 1.0 42.1
trnY-GUA-rpoB_p2 AACGAATTTACAGTCCGCCC TCTGATTAAATCCAGGTATTGTGG 50.0 37.5 58.6 56.5 1158-1362 bp 1.0 42.1
trnY-GUA-rpoB_p3 CCATTAACCACTCGGGCATC TCTGATTAAATCCAGGTATTGTGG 55.0 37.5 58.7 56.5 1138-1342 bp 1.0 42.1
trnY-GUA-rpoB_p4 AGATTGGCAAATTGTTGGGC TCTGATTAAATCCAGGTATTGTGG 45.0 37.5 57.5 56.5 1212-1416 bp 1.0 42.0
trnY-GUA-rpoB_p5 GATTGGCAAATTGTTGGGCC TCTGATTAAATCCAGGTATTGTGG 50.0 37.5 58.5 56.5 1211-1415 bp 1.0 42.0