Hotspot

psbI-trnS-GCU

LSC · 8202-8347 · 146 bp

Back to Baccharis

Pi 0.0365

MarkerSeek score 75.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 146
variable_sites: 8
pi: 0.0365
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	262-264 bp	1.0	74.1
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	210-212 bp	1.0	72.4
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	262-264 bp	1.0	71.8
psbI-trnS-GCU_p4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	52.4	50.0	57.7	58.1	281-283 bp	1.0	70.8
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.1	58.6	253-255 bp	1.0	70.0