Hotspot

tRNA-Leu-tRNA-Asn

SSC · 108776-126832 · 18057 bp

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Pi 0.0259
MarkerSeek score 63.60
Primer available yes
Label tRNA-Leu-tRNA-Asn

Feature Metadata

label_name
tRNA-Leu-tRNA-Asn
feature_type
igs
region
SSC
length_bp
18057
variable_sites
871
pi
0.0259
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Leu-tRNA-Asn_p1 GGATTTCCACTTCCACCGAG TTTCTGCGAGATGGGCATAC 55.0 50.0 58.3 58.0 825-975 bp 1.0 89.2
tRNA-Leu-tRNA-Asn_p2 GGATTTCCACTTCCACCGAG ATTTTCTGCGAGATGGGCAT 55.0 45.0 58.3 57.9 827-977 bp 1.0 89.0
tRNA-Leu-tRNA-Asn_p3 GGATTTCCACTTCCACCGAG CTGCCTTGGATGCCCATAAT 55.0 50.0 58.3 58.0 1115-1265 bp 1.0 88.6
tRNA-Leu-tRNA-Asn_p4 TCGGTACTAGACGATGAGCA CTGCCTTGGATGCCCATAAT 50.0 50.0 57.7 58.0 1311-1461 bp 1.0 87.6
tRNA-Leu-tRNA-Asn_p5 GGATTTCCACTTCCACCGAG CCAGATAGTTATGCTCCCGC 55.0 55.0 58.3 57.9 1281-1335 bp 0.6 68.7