Hotspot

tRNA-Pro-tRNA-Leu

SSC · 108527-108695 · 169 bp

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Pi 0.0556
MarkerSeek score 80.40
Primer available yes
Label tRNA-Pro-tRNA-Leu

Feature Metadata

label_name
tRNA-Pro-tRNA-Leu
feature_type
igs
region
SSC
length_bp
169
variable_sites
17
pi
0.0556
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Pro-tRNA-Leu_p1 GATTCGAACCTGTGACCTCC TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.0 57.9 270-313 bp 1.0 84.2
tRNA-Pro-tRNA-Leu_p2 GGATTCGAACCTGTGACCTC TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.0 57.9 271-314 bp 1.0 84.1
tRNA-Pro-tRNA-Leu_p3 GATTCGAACCTGTGACCTCC CAGCGTGTCTACCAGTTTCA 55.0 50.0 58.0 57.9 261-304 bp 1.0 84.1
tRNA-Pro-tRNA-Leu_p4 GGATTCGAACCTGTGACCTC CAGCGTGTCTACCAGTTTCA 55.0 50.0 58.0 57.9 262-305 bp 1.0 84.1
tRNA-Pro-tRNA-Leu_p5 GATTCGAACCTGTGACCTCC AGCGTGTCTACCAGTTTCAC 55.0 50.0 58.0 57.8 260-303 bp 1.0 84.1