Hotspot

tRNA-Asn-tRNA-Pro

SSC · 103979-108452 · 4474 bp

Back to Azolla

Pi 0.0358
MarkerSeek score 72.90
Primer available yes
Label tRNA-Asn-tRNA-Pro

Feature Metadata

label_name
tRNA-Asn-tRNA-Pro
feature_type
igs
region
SSC
length_bp
4474
variable_sites
293
pi
0.0358
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Asn-tRNA-Pro_p1 CAAGAGCTCTTTCCTCAGCC AACTCTGGCTATGTCCAAGC 55.0 50.0 58.3 57.6 819-848 bp 0.6 69.4
tRNA-Asn-tRNA-Pro_p2 CAAGAGCTCTTTCCTCAGCC ACTCTGGCTATGTCCAAGCT 55.0 50.0 58.3 58.4 818-847 bp 0.6 69.3
tRNA-Asn-tRNA-Pro_p3 AACGAGAAAGCTTCTGGACC AACTCTGGCTATGTCCAAGC 50.0 50.0 57.8 57.6 783 bp 0.2 43.3
tRNA-Asn-tRNA-Pro_p4 AACGAGAAAGCTTCTGGACC ACTCTGGCTATGTCCAAGCT 50.0 50.0 57.8 58.4 782 bp 0.2 43.3
tRNA-Asn-tRNA-Pro_p5 GAGAAAGCTTCTGGACCGAA AACTCTGGCTATGTCCAAGC 50.0 50.0 57.5 57.6 780 bp 0.2 41.1