Hotspot

tRNA-Pro-tRNA-Ile

LSC · 63546-83198 · 19653 bp

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Pi 0.0206
MarkerSeek score 58.30
Primer available yes
Label tRNA-Pro-tRNA-Ile

Feature Metadata

label_name
tRNA-Pro-tRNA-Ile
feature_type
igs
region
LSC
length_bp
19653
variable_sites
750
pi
0.0206
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Pro-tRNA-Ile_p1 GCTAATCTCCCTTCCTTGGC CATCACCATCAGACACAGGG 55.0 55.0 58.0 58.0 1054-1082 bp 1.0 86.9
tRNA-Pro-tRNA-Ile_p2 GATCCAGCCTTTCCTAAGCC CATCACCATCAGACACAGGG 55.0 55.0 58.0 58.0 1168-1196 bp 1.0 86.6
tRNA-Pro-tRNA-Ile_p3 GATCCAGCCTTTCCTAAGCC AAAAGATGTTGACCTGCCGT 55.0 45.0 58.0 58.0 1243 bp 0.2 47.6
tRNA-Pro-tRNA-Ile_p4 GCTAATCTCCCTTCCTTGGC AAAAGATGTTGACCTGCCGT 55.0 45.0 58.0 58.0 1129 bp 0.2 47.6
tRNA-Pro-tRNA-Ile_p5 CGTGTAGATCCAGCCTTTCC AAAAGATGTTGACCTGCCGT 55.0 45.0 58.1 58.0 1249 bp 0.2 47.4