Hotspot

tRNA-Ser-tRNA-Leu

LSC · 44853-46444 · 1592 bp

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Pi 0.0319
MarkerSeek score 74.60
Primer available yes
Label tRNA-Ser-tRNA-Leu

Feature Metadata

label_name
tRNA-Ser-tRNA-Leu
feature_type
igs
region
LSC
length_bp
1592
variable_sites
95
pi
0.0319
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Ser-tRNA-Leu_p1 CGAGGGGTTTAAGGTGTAGC CTGAATCTGGGAGCTGTCAC 55.0 55.0 58.0 58.0 1751-1764 bp 1.0 85.3
tRNA-Ser-tRNA-Leu_p2 CGAGGGGTTTAAGGTGTAGC TCGATCCGATTGAGTCCGTA 55.0 50.0 58.0 58.0 1708-1721 bp 1.0 85.3
tRNA-Ser-tRNA-Leu_p3 CGAGGGGTTTAAGGTGTAGC GATCCGATTGAGTCCGTAGC 55.0 55.0 58.0 57.9 1706-1719 bp 1.0 85.2
tRNA-Ser-tRNA-Leu_p4 GCTGTTTACCGAGGGTTCAA CTGAATCTGGGAGCTGTCAC 50.0 55.0 58.1 58.0 1710-1723 bp 1.0 84.7
tRNA-Ser-tRNA-Leu_p5 CGAGGGGTTTAAGGTGTAGC GTCCGTAGCGTCTACCATTC 55.0 55.0 58.0 57.9 1695-1708 bp 1.0 84.7