Hotspot

tRNA-Cys-tRNA-Gly

LSC · 30608-30896 · 289 bp

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Pi 0.0705
MarkerSeek score 82.70
Primer available yes
Label tRNA-Cys-tRNA-Gly

Feature Metadata

label_name
tRNA-Cys-tRNA-Gly
feature_type
igs
region
LSC
length_bp
289
variable_sites
37
pi
0.0705
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Cys-tRNA-Gly_p1 CTCGAACTGGGGAATCAAGG GTTCGATTCCCGCTATCCG 55.0 57.9 58.0 58.2 360-368 bp 1.0 80.7
tRNA-Cys-tRNA-Gly_p2 CTCGAACTGGGGAATCAAGG GGGTTCGATTCCCGCTATC 55.0 57.9 58.0 57.8 362-370 bp 1.0 80.5
tRNA-Cys-tRNA-Gly_p3 CTCGAACTGGGGAATCAAGG GGTTCGATTCCCGCTATCC 55.0 57.9 58.0 57.8 361-369 bp 1.0 80.5
tRNA-Cys-tRNA-Gly_p4 AGACTCGAACTGGGGAATCA GTTCGATTCCCGCTATCCG 50.0 57.9 58.1 58.2 363-371 bp 1.0 80.4
tRNA-Cys-tRNA-Gly_p5 TTTAGTAGGCGACACCCAGA GTTCGATTCCCGCTATCCG 50.0 57.9 58.1 58.2 380-388 bp 1.0 79.9