Hotspot

tRNA-Tyr-tRNA-Glu

LSC · 28877-29055 · 179 bp

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Pi 0.0367
MarkerSeek score 65.80
Primer available yes
Label tRNA-Tyr-tRNA-Glu

Feature Metadata

label_name
tRNA-Tyr-tRNA-Glu
feature_type
igs
region
LSC
length_bp
179
variable_sites
12
pi
0.0367
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Tyr-tRNA-Glu_p1 GTCCCCATTAACCACTCGAG CGTCTAGAGGCCTAGGACAA 55.0 55.0 57.7 57.9 269-273 bp 1.0 84.5
tRNA-Tyr-tRNA-Glu_p2 CCCATTAACCACTCGAGCAT CGTCTAGAGGCCTAGGACAA 50.0 55.0 57.7 57.9 266-270 bp 1.0 84.4
tRNA-Tyr-tRNA-Glu_p3 GTCCCCATTAACCACTCGAG GAGGCCTAGGACAACTCTCT 55.0 55.0 57.7 57.8 263-267 bp 1.0 83.9
tRNA-Tyr-tRNA-Glu_p4 GTCCCCATTAACCACTCGAG AGAGGCCTAGGACAACTCTC 55.0 55.0 57.7 57.8 264-268 bp 1.0 83.8
tRNA-Tyr-tRNA-Glu_p5 CCCATTAACCACTCGAGCAT GAGGCCTAGGACAACTCTCT 50.0 55.0 57.7 57.8 260-264 bp 1.0 83.8