Hotspot

tRNA-Gly-tRNA-Gly

LSC · 9930-10784 · 855 bp

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Pi 0.0261
MarkerSeek score 66.80
Primer available yes
Label tRNA-Gly-tRNA-Gly

Feature Metadata

label_name
tRNA-Gly-tRNA-Gly
feature_type
igs
region
LSC
length_bp
855
variable_sites
42
pi
0.0261
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Gly-tRNA-Gly_p1 TTGTGGCGGGTATGGTTTAG CCCGCATCATTAGCTTGGAA 50.0 50.0 57.9 58.3 910-932 bp 1.0 81.0
tRNA-Gly-tRNA-Gly_p2 TTAGACAGAGGACCCGTCTG CCCGCATCATTAGCTTGGAA 55.0 50.0 58.2 58.3 945-967 bp 1.0 80.5
tRNA-Gly-tRNA-Gly_p3 AGAGGACCCGTCTGTGTAAT CCCGCATCATTAGCTTGGAA 50.0 50.0 57.8 58.3 939-961 bp 1.0 80.0
tRNA-Gly-tRNA-Gly_p4 TTGTGGCGGGTATGGTTTAG ATCGAACCCGCATCATTAGC 50.0 50.0 57.9 58.5 916-938 bp 1.0 79.7
tRNA-Gly-tRNA-Gly_p5 TTAGACAGAGGACCCGTCTG ATCGAACCCGCATCATTAGC 55.0 50.0 58.2 58.5 951-973 bp 1.0 79.1