Hotspot

tRNA-Gln-tRNA-Ser

LSC · 7825-9017 · 1193 bp

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Pi 0.0317
MarkerSeek score 72.60
Primer available yes
Label tRNA-Gln-tRNA-Ser

Feature Metadata

label_name
tRNA-Gln-tRNA-Ser
feature_type
igs
region
LSC
length_bp
1193
variable_sites
73
pi
0.0317
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Gln-tRNA-Ser_p1 GATCAAAACCCGCTGCCTTA GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.5 58.2 1251-1272 bp 1.0 78.9
tRNA-Gln-tRNA-Ser_p2 GATCAAAACCCGCTGCCTTA TAACGCGTCGGATTGCTAAT 50.0 45.0 58.5 57.8 1296-1317 bp 1.0 78.8
tRNA-Gln-tRNA-Ser_p3 GATCAAAACCCGCTGCCTTA CTAACGCGTCGGATTGCTAA 50.0 50.0 58.5 58.4 1297-1318 bp 1.0 76.9
tRNA-Gln-tRNA-Ser_p4 GATCAAAACCCGCTGCCTTA TTTTGCTTGGAGAGATGGCC 50.0 50.0 58.5 58.5 1324-1345 bp 1.0 76.7
tRNA-Gln-tRNA-Ser_p5 CGAATGACGGGATCAAAACC TAACGCGTCGGATTGCTAAT 50.0 45.0 57.2 57.8 1306-1327 bp 1.0 76.4