Hotspot

ycf1

SSC · 129359-134836 · 5478 bp

Back to Azadirachta

Pi 0.00767

MarkerSeek score 49.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 42
pi: 0.00767
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTTATGTCCGATCCCGTA	TACCAACCAAACTGCTTTTT	40.0	35.0	54.2	53.8	261 bp	1.0	41.5
ycf1_p2	TTTTATGTCCGATCCCGTAT	TACCAACCAAACTGCTTTTT	40.0	35.0	53.9	53.8	260 bp	1.0	41.5
ycf1_p3	TTTTTATGTCCGATCCCGTAT	TACCAACCAAACTGCTTTTT	38.1	35.0	54.8	53.8	261 bp	1.0	41.5
ycf1_p4	TTTTTATGTCCGATCCCGT	TACCAACCAAACTGCTTTTT	42.1	35.0	54.2	53.8	261 bp	1.0	41.5
ycf1_p5	TTTTTATGTCCGATCCCGTA	ACCAACCAAACTGCTTTTT	40.0	36.8	54.2	53.7	260 bp	1.0	41.5