Hotspot

petD

LSC · 81590-82114 · 525 bp

Back to Azadirachta

Pi 0.00571

MarkerSeek score 49.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 525
variable_sites: 3
pi: 0.00571
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GCTCGAGCCGGATGATAAAA	TCCCTAGATACACGCGTCAT	50.0	50.0	58.1	58.0	595 bp	1.0	79.1
petD_p2	GCTCGAGCCGGATGATAAAA	TTCTCCCTAGATACACGCGT	50.0	50.0	58.1	58.0	598 bp	1.0	78.9
petD_p3	GCTCGAGCCGGATGATAAAA	CCCTAGATACACGCGTCATG	50.0	55.0	58.1	57.9	594 bp	1.0	78.9
petD_p4	TTAATTCTTGCTCGAGCCGG	TCCCTAGATACACGCGTCAT	50.0	50.0	58.3	58.0	604 bp	1.0	77.4
petD_p5	TTAATTCTTGCTCGAGCCGG	TTCTCCCTAGATACACGCGT	50.0	50.0	58.3	58.0	607 bp	1.0	77.2