Hotspot

ndhI-ndhA

SSC · 125105-125192 · 88 bp

Back to Azadirachta

Pi 0.0341

MarkerSeek score 71.50

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 88
variable_sites: 3
pi: 0.0341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGCAGCTCGTATTGTTTGGT	TTCCCAACTTCTTTCGCTGT	45.0	45.0	58.1	57.7	167 bp	1.0	77.8
ndhI-ndhA_p2	ACCTTGCAGCTCGTATTGTT	TTCCCAACTTCTTTCGCTGT	45.0	45.0	57.8	57.7	171 bp	1.0	77.2
ndhI-ndhA_p3	GCAGCTCGTATTGTTTGGTG	TTCCCAACTTCTTTCGCTGT	50.0	45.0	57.8	57.7	166 bp	1.0	76.7
ndhI-ndhA_p4	TTTGCCCAATGTACCTTGCA	TTCCCAACTTCTTTCGCTGT	45.0	45.0	58.3	57.7	183 bp	1.0	76.3
ndhI-ndhA_p5	CTTTGCCCAATGTACCTTGC	TTCCCAACTTCTTTCGCTGT	50.0	45.0	57.6	57.7	184 bp	1.0	75.6