Hotspot

rpl32-trnL-UAG

SSC · 118463-119562 · 1100 bp

Back to Azadirachta

Pi 0.0173

MarkerSeek score 57.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1100
variable_sites: 19
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCGGGAATTCAAAAAGTTT	AGCGTGTCTACCGATTTCAC	35.0	50.0	53.9	58.0	1171-1174 bp	1.0	48.3
rpl32-trnL-UAG_p2	ACCGGGAATTCAAAAAGTTT	CAGCGTGTCTACCGATTTCA	35.0	50.0	53.9	58.0	1172-1175 bp	1.0	48.3
rpl32-trnL-UAG_p3	ACCGGGAATTCAAAAAGTTT	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	53.9	57.9	1181-1184 bp	1.0	47.4
rpl32-trnL-UAG_p4	ACCGGGAATTCAAAAAGTTTT	AGCGTGTCTACCGATTTCAC	33.3	50.0	54.7	58.0	1171-1174 bp	1.0	47.4
rpl32-trnL-UAG_p5	ACCGGGAATTCAAAAAGTTTT	CAGCGTGTCTACCGATTTCA	33.3	50.0	54.7	58.0	1172-1175 bp	1.0	47.3