Hotspot

ycf4-cemA

LSC · 64255-65195 · 941 bp

Back to Azadirachta

Pi 0.0225

MarkerSeek score 66.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 941
variable_sites: 21
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	GAGATCGACCAGGGCAAAAA	45.0	50.0	58.1	58.2	1051-1060 bp	1.0	80.1
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	TGAGAGAGAGATCGACCAGG	45.0	55.0	58.1	57.7	1058-1067 bp	1.0	78.8
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GAGAGAGAGATCGACCAGGG	45.0	60.0	58.1	58.4	1057-1066 bp	1.0	78.3
ycf4-cemA_p4	GGCCATTCCCTTGAATCGTA	GAGATCGACCAGGGCAAAAA	50.0	50.0	57.7	58.2	1118-1127 bp	1.0	78.0
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	AGATCGACCAGGGCAAAAAT	45.0	45.0	58.1	57.5	1050-1059 bp	1.0	77.4