Hotspot

trnS-UGA-psbZ

LSC · 38511-38877 · 367 bp

Back to Azadirachta

Pi 0.014

MarkerSeek score 55.90

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 5
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	426-435 bp	1.0	74.2
trnS-UGA-psbZ_p2	CTATCAACCACTCGGCCATC	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.1	57.0	415-424 bp	1.0	73.7
trnS-UGA-psbZ_p3	TTCACCGAGCAAAAGGAGAG	TGCAAAAACAGCCAATTGGA	50.0	40.0	57.8	57.0	496-506 bp	1.0	73.1
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	GGAGAAGCAAATACAACGGG	55.0	50.0	58.1	56.8	482-491 bp	1.0	72.2
trnS-UGA-psbZ_p5	CACCGAGCAAAAGGAGAGAG	TGCAAAAACAGCCAATTGGA	55.0	40.0	58.3	57.0	494-504 bp	1.0	72.2