Hotspot

psbC-trnS-UGA

LSC · 38172-38417 · 246 bp

Back to Azadirachta

Pi 0.0329

MarkerSeek score 69.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 8
pi: 0.0329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TGATCGTGAGCTTGAACCTG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.9	58.1	366-370 bp	1.0	81.5
psbC-trnS-UGA_p2	GATCGTGAGCTTGAACCTGT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.9	58.1	365-369 bp	1.0	81.5
psbC-trnS-UGA_p3	CGTGAGCTTGAACCTGTTCT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	362-366 bp	1.0	81.1
psbC-trnS-UGA_p4	TCGTGAGCTTGAACCTGTTC	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	363-367 bp	1.0	81.1
psbC-trnS-UGA_p5	TGATCGTGAGCTTGAACCTG	GATGGCCGAGTGGTTGATAG	50.0	55.0	57.9	58.1	377-381 bp	1.0	80.8