Hotspot

rpoC2-rpoC1

LSC · 22599-22776 · 178 bp

Back to Azadirachta

Pi 0.0281

MarkerSeek score 68.70

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 5
pi: 0.0281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	TGTGAAAGACCAAACTCGCC	TACAAGGTTTTTGTCGGGCC	50.0	50.0	58.7	58.7	247 bp	1.0	70.6
rpoC2-rpoC1_p2	TGTGAAAGACCAAACTCGCC	GGCCTGCTCATATGGTACCT	50.0	55.0	58.7	58.9	231 bp	1.0	68.7
rpoC2-rpoC1_p3	TGAAAGACCAAACTCGCCC	TACAAGGTTTTTGTCGGGCC	52.6	50.0	58.0	58.7	245 bp	1.0	68.1
rpoC2-rpoC1_p4	ATGCCATTCCGAAGTGATCT	TACAAGGTTTTTGTCGGGCC	45.0	50.0	57.0	58.7	316 bp	1.0	67.7
rpoC2-rpoC1_p5	TTGTGAAAGACCAAACTCGC	TACAAGGTTTTTGTCGGGCC	45.0	50.0	56.9	58.7	248 bp	1.0	67.2