Hotspot

trnK-UUU-rps16

LSC · 4448-5447 · 1000 bp

Back to Azadirachta

Pi 0.0417

MarkerSeek score 71.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 40
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGTACTTAAAAGCCGAG	CGAGCCGTACGAAGAGAAAA	50.0	50.0	58.0	58.0	1313-1342 bp	1.0	84.9
trnK-UUU-rps16_p2	AGCCGTACTTAAAAGCCGAG	TTTTCTCGAGCCGTACGAAG	50.0	50.0	58.0	58.0	1319-1348 bp	1.0	84.9
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	CGAGCCGTACGAAGAGAAAA	55.0	50.0	58.0	58.0	1397-1426 bp	1.0	84.5
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	TTTTCTCGAGCCGTACGAAG	55.0	50.0	58.0	58.0	1403-1432 bp	1.0	84.5
trnK-UUU-rps16_p5	AGCCGTACTTAAAAGCCGAG	GGTGTGTTGCTGCCATTTTT	50.0	45.0	58.0	58.0	1703-1742 bp	1.0	84.2