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psbA

LSC · 87-1148 · 1062 bp

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Pi 0.00439

MarkerSeek score 49.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 7
pi: 0.00439
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ATTCCTATATGTTAGAGAAAAACC	AATACGTGTGCTTGGGAGTC	29.2	50.0	52.3	57.9	1136 bp	1.0	40.5
psbA_p2	AATTCCTATATGTTAGAGAAAAACC	AATACGTGTGCTTGGGAGTC	28.0	50.0	53.1	57.9	1137 bp	1.0	40.5
psbA_p3	AAATTCCTATATGTTAGAGAAAAACC	AATACGTGTGCTTGGGAGTC	26.9	50.0	53.9	57.9	1138 bp	1.0	40.5
psbA_p4	ATTCCTATATGTTAGAGAAAAACC	TGGGAGTCCTTGCAATTTGA	29.2	45.0	52.3	57.3	1124 bp	1.0	40.5
psbA_p5	ATTCCTATATGTTAGAGAAAAACC	GCTTGGGAGTCCTTGCAATT	29.2	50.0	52.3	58.7	1127 bp	1.0	40.5