Hotspot

trnL-CAA-ycf15

IRa · 132961-133736 · 776 bp

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Pi 0.00258

MarkerSeek score 60.40

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 776
variable_sites: 3
pi: 0.00258
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	TCCCTTTGACAAATCGGGTG	50.0	50.0	58.0	58.1	912 bp	1.0	79.4
trnL-CAA-ycf15_p2	TGAAATGGTAGACACGCGAG	TCCCTTTGACAAATCGGGTG	50.0	50.0	58.0	58.1	914 bp	1.0	79.4
trnL-CAA-ycf15_p3	TGGTGAAATGGTAGACACGC	TCCCTTTGACAAATCGGGTG	50.0	50.0	58.2	58.1	917 bp	1.0	77.9
trnL-CAA-ycf15_p4	GAAATGGTAGACACGCGAGA	TCCCTTTGACAAATCGGGTG	50.0	50.0	57.7	58.1	913 bp	1.0	77.3
trnL-CAA-ycf15_p5	GTTCGAGTCCTCTTCAAGGC	TCCCTTTGACAAATCGGGTG	55.0	50.0	58.3	58.1	864 bp	1.0	77.2