Hotspot

rpl32-trnL-UAG

SSC · 107102-107612 · 511 bp

Back to Axonopus

Pi 0.0157

MarkerSeek score 66.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 511
variable_sites: 12
pi: 0.0157
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAACGAGGATCCAAAACCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.8	57.9	603-610 bp	1.0	74.5
rpl32-trnL-UAG_p2	GCAACGAGGATCCAAAACCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.8	57.8	596-603 bp	1.0	74.1
rpl32-trnL-UAG_p3	GCAACGAGGATCCAAAACCA	GCTTGAGCACTGCTTCCTAA	50.0	50.0	58.8	58.2	617-624 bp	1.0	74.0
rpl32-trnL-UAG_p4	GCAACGAGGATCCAAAACCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.8	57.8	602-609 bp	1.0	74.0
rpl32-trnL-UAG_p5	GCAACGAGGATCCAAAACCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.8	57.8	601-608 bp	1.0	74.0