Hotspot

trnR-UCU-trnfM-CAU

LSC · 37379-37547 · 169 bp

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Pi 0.0316

MarkerSeek score 60.00

Primer available yes

Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name: trnR-UCU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 8
pi: 0.0316
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnfM-CAU_p1	AGAAGACCTCTGTCCTATCCA	CAAAGCTCATAACCTTGGGG	47.6	50.0	56.9	56.4	238-239 bp	1.0	53.3
trnR-UCU-trnfM-CAU_p2	AGGTTTAGAAGACCTCTGTCCT	CAAAGCTCATAACCTTGGGG	45.5	50.0	57.9	56.4	244-245 bp	1.0	52.6
trnR-UCU-trnfM-CAU_p3	AGAAGACCTCTGTCCTATCCA	ACCTTGGGGTTACGGGTT	47.6	55.6	56.9	58.0	227-228 bp	1.0	50.3
trnR-UCU-trnfM-CAU_p4	AGGTTTAGAAGACCTCTGTCCT	ACCTTGGGGTTACGGGTT	45.5	55.6	57.9	58.0	233-234 bp	1.0	49.6
trnR-UCU-trnfM-CAU_p5	AGAAGACCTCTGTCCTATCCA	TTGGGGTTACGGGTTCGA	47.6	55.6	56.9	58.1	224-225 bp	1.0	49.5