Hotspot

trnC-GCA-rpoB

LSC · 19427-20571 · 1145 bp

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Pi 0.00817

MarkerSeek score 59.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1145
variable_sites: 14
pi: 0.00817
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	GCGGACATTCCCTCATTTCT	50.0	50.0	58.1	58.0	1220-1232 bp	1.0	80.1
trnC-GCA-rpoB_p2	CTGGGGGTAAAGGATTTGCA	GCGGACATTCCCTCATTTCT	50.0	50.0	57.8	58.0	1228-1240 bp	1.0	78.8
trnC-GCA-rpoB_p3	TGGGGGTAAAGGATTTGCAG	GCGGACATTCCCTCATTTCT	50.0	50.0	57.8	58.0	1227-1239 bp	1.0	78.8
trnC-GCA-rpoB_p4	ACTGGGGGTAAAGGATTTGC	GCGGACATTCCCTCATTTCT	50.0	50.0	57.8	58.0	1229-1241 bp	1.0	78.8
trnC-GCA-rpoB_p5	GGGGGTAAAGGATTTGCAGT	GCGGACATTCCCTCATTTCT	50.0	50.0	57.8	58.0	1226-1238 bp	1.0	78.8