Hotspot

trnG-UCC-trnT-GGU

LSC · 13594-15251 · 1658 bp

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Pi 0.00684

MarkerSeek score 62.80

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1658
variable_sites: 17
pi: 0.00684
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	38.1	45.0	55.5	56.7	1778-1780 bp	1.0	41.7
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	1748-1750 bp	1.0	41.5
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	1744-1746 bp	1.0	40.8
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	38.1	47.6	55.5	57.5	1745-1747 bp	1.0	40.8
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	39.1	45.0	57.3	56.7	1780-1782 bp	1.0	40.8