Hotspot

ycf2

IRb · 86049-92864 · 6816 bp

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Pi 0.0107

MarkerSeek score 58.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6816
variable_sites: 73
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TTCTTTTGTCCCTTCCAGGC	TCATGGATCTCTCGGTTCGA	50.0	50.0	58.0	57.9	842-851 bp	1.0	82.9
ycf2_p2	TTCTTTTGTCCCTTCCAGGC	TTGAAAACGGCTCTTCTGCT	50.0	45.0	58.0	58.0	943-952 bp	1.0	82.7
ycf2_p3	TTCTTTTGTCCCTTCCAGGC	TAGATGTGGATCTCGGACCC	50.0	55.0	58.0	58.0	1152-1161 bp	1.0	82.4
ycf2_p4	ATGACCAGAACAGGGGAAGA	TAGATGTGGATCTCGGACCC	50.0	55.0	58.0	58.0	952 bp	0.5	59.6
ycf2_p5	TGACCAGAACAGGGGAAGAT	TAGATGTGGATCTCGGACCC	50.0	55.0	58.0	58.0	951 bp	0.5	59.6