Hotspot

ndhJ-ndhK

LSC · 49237-49336 · 100 bp

Back to Avicennia

Pi 0.03

MarkerSeek score 69.80

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 100
variable_sites: 3
pi: 0.03
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CTAGCCAAGCAGACAAACGA	GCCATCGACTTCAGAGATCC	50.0	55.0	58.2	57.9	207 bp	1.0	78.3
ndhJ-ndhK_p2	CTAGCCAAGCAGACAAACGA	TATCAACCGCCATCGACTTC	50.0	50.0	58.2	57.8	215 bp	1.0	77.7
ndhJ-ndhK_p3	CTAGCCAAGCAGACAAACGA	CTATCAACCGCCATCGACTT	50.0	50.0	58.2	57.8	216 bp	1.0	77.7
ndhJ-ndhK_p4	CTAGCCAAGCAGACAAACGA	CTCTATCAACCGCCATCGAC	50.0	55.0	58.2	58.2	218 bp	1.0	77.6
ndhJ-ndhK_p5	CTAGCCAAGCAGACAAACGA	CGCCATCGACTTCAGAGATC	50.0	55.0	58.2	58.2	208 bp	1.0	77.6