Hotspot

trnT-GGU-psbD

LSC · 31787-33154 · 1368 bp

Back to Avicennia

Pi 0.0157

MarkerSeek score 69.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1368
variable_sites: 21
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1503-1528 bp	1.0	81.3
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1506-1531 bp	1.0	81.1
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1493-1518 bp	1.0	80.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1496-1521 bp	1.0	80.0
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CCCTTCGTAACCAGTCATCC	55.0	55.0	58.1	57.7	1477-1502 bp	1.0	79.7