Hotspot

atpH-atpI

LSC · 13793-14793 · 1001 bp

Back to Avicennia

Pi 0.0131

MarkerSeek score 66.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 13
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1135-1139 bp	1.0	78.8
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1152-1156 bp	1.0	77.9
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.3	58.3	1147-1151 bp	1.0	77.5
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1087-1091 bp	1.0	76.8
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	57.6	58.3	1148-1152 bp	1.0	76.3