Hotspot

trnR-UCU-atpA

LSC · 10246-10351 · 106 bp

Back to Avicennia

Pi 0.0388

MarkerSeek score 65.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 106
variable_sites: 4
pi: 0.0388
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAAAATCGGAATGAACGGCG	CGGCTTCAGGAACAAGCATA	45.0	50.0	57.7	58.3	213-216 bp	1.0	78.5
trnR-UCU-atpA_p2	GAACGGCGTCCATTGTCTAA	CGGCTTCAGGAACAAGCATA	50.0	50.0	58.3	58.3	201-204 bp	1.0	78.4
trnR-UCU-atpA_p3	ACGGCGTCCATTGTCTAATG	CGGCTTCAGGAACAAGCATA	50.0	50.0	58.3	58.3	199-202 bp	1.0	77.9
trnR-UCU-atpA_p4	AACGGCGTCCATTGTCTAAT	CGGCTTCAGGAACAAGCATA	45.0	50.0	57.6	58.3	200-203 bp	1.0	77.5
trnR-UCU-atpA_p5	AAAAATCGGAATGAACGGCG	TAAACCGGCTTCAGGAACAA	45.0	45.0	57.7	57.4	218-221 bp	1.0	75.4