Hotspot

trnG-CGA-trnR-UCU

LSC · 9997-10173 · 177 bp

Back to Avicennia

Pi 0.0409

MarkerSeek score 67.70

Primer available yes

Label trnG-CGA-trnR-UCU

Feature Metadata

label_name: trnG-CGA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 177
variable_sites: 7
pi: 0.0409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-CGA-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	251-257 bp	1.0	67.4
trnG-CGA-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	254-260 bp	1.0	67.2
trnG-CGA-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	257-263 bp	1.0	66.7
trnG-CGA-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	260-266 bp	1.0	66.4
trnG-CGA-trnR-UCU_p5	CTAACGATGCGGGTTCGATT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	240-246 bp	1.0	64.4