Hotspot

trnH-GUG-psbA

LSC · 352-759 · 408 bp

Back to Avicennia

Pi 0.0661

MarkerSeek score 78.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 25
pi: 0.0661
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	445-474 bp	1.0	86.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	459-488 bp	1.0	86.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	458-487 bp	1.0	86.6
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	460-489 bp	1.0	86.5
trnH-GUG-psbA_p5	GGGAGTCATTATCATGGGCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.2	57.9	543 bp	0.5	57.9