Hotspot

psaC

SSC · 119373-119618 · 246 bp

Back to Averrhoa

Pi 0

MarkerSeek score 19.50

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CTAGAAAAACCCGTGCTCGA	GATTGATCAGGGGCCGAAAT	50.0	50.0	57.9	58.0	816-824 bp	1.0	81.5
psaC_p2	TCTTTCCTCCCCACATGGAT	GATTGATCAGGGGCCGAAAT	50.0	50.0	58.0	58.0	1314-1322 bp	1.0	81.4
psaC_p3	TGGACTAGAAAAACCCGTGC	GATTGATCAGGGGCCGAAAT	50.0	50.0	58.1	58.0	820-828 bp	1.0	81.2
psaC_p4	AGCACGGGTTTTTGTCGATA	GATTGATCAGGGGCCGAAAT	45.0	50.0	57.8	58.0	759-765 bp	1.0	79.1
psaC_p5	TATGTTTTCGAGCACGGGTT	GATTGATCAGGGGCCGAAAT	45.0	50.0	57.8	58.0	769 bp	0.5	58.5