Hotspot

ndhF

SSC · 112860-115073 · 2214 bp

Back to Averrhoa

Pi 0.00727

MarkerSeek score 47.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 16
pi: 0.00727
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGGATCCGGACAAAATCGA	TCAGTTATGTATGGGCTGTCT	50.0	42.9	57.4	56.1	2909-2939 bp	1.0	54.8
ndhF_p2	ACAAGAGGAATCCACCGAAG	TCAGTTATGTATGGGCTGTCT	50.0	42.9	57.2	56.1	2964-2994 bp	1.0	53.4
ndhF_p3	GAGGATCCGGACAAAATCGA	TCAGTTATGTATGGGCTGTCTT	50.0	40.9	57.4	56.9	2909-2939 bp	1.0	52.8
ndhF_p4	ACAAGAGGAATCCACCGAAG	TCAGTTATGTATGGGCTGTCTT	50.0	40.9	57.2	56.9	2964-2994 bp	1.0	51.3
ndhF_p5	GAAACAAGAGGAATCCACCGA	TCAGTTATGTATGGGCTGTCT	47.6	42.9	57.9	56.1	2967-2997 bp	1.0	51.1