Hotspot

ndhD-psaC

SSC · 119249-119372 · 124 bp

Back to Averrhoa

Pi 0.145

MarkerSeek score 73.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 124
variable_sites: 18
pi: 0.145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TCTTTCCTCCCCACATGGAT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	743-745 bp	1.0	82.1
ndhD-psaC_p2	TCTTTCCTCCCCACATGGAT	TGTGAATCTGCCTGTCCAAC	50.0	50.0	58.0	58.1	704-706 bp	1.0	81.6
ndhD-psaC_p3	TCTTTCCTCCCCACATGGAT	GATTGATCAGGGGCCGAAAT	50.0	50.0	58.0	58.0	1314-1322 bp	1.0	81.4
ndhD-psaC_p4	TGTAATGTGCTTCTCCGTGG	TCTGCTCCAAGAACAGAGGA	50.0	50.0	57.9	58.0	998-1000 bp	1.0	80.9
ndhD-psaC_p5	TGTAATGTGCTTCTCCGTGG	GATTGATCAGGGGCCGAAAT	50.0	50.0	57.9	58.0	1569-1577 bp	1.0	80.5