Hotspot

rpl32-trnL-UAG

SSC · 115897-116314 · 418 bp

Back to Averrhoa

Pi 0.0412

MarkerSeek score 72.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 418
variable_sites: 17
pi: 0.0412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGTATTGGACAGCGCTAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	542-548 bp	1.0	82.9
rpl32-trnL-UAG_p2	GGGTATTGGACAGCGCTAAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	543-549 bp	1.0	82.9
rpl32-trnL-UAG_p3	GGGTATTGGACAGCGCTAAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	552-558 bp	1.0	81.9
rpl32-trnL-UAG_p4	GGGTATTGGACAGCGCTAAA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	550-556 bp	1.0	81.4
rpl32-trnL-UAG_p5	GGGTATTGGACAGCGCTAAA	TTAGAAGATGCTATGCCGCC	50.0	50.0	58.0	57.8	606-612 bp	1.0	81.3