Hotspot

psaJ-rpl33

LSC · 69879-70381 · 503 bp

Back to Averrhoa

Pi 0.0596

MarkerSeek score 74.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 503
variable_sites: 30
pi: 0.0596
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCGTTTATTTCCGGATGCGT	TCGGATATCTTTGCCCTTGG	45.0	50.0	58.0	57.4	573-580 bp	1.0	79.0
psaJ-rpl33_p2	CATATCTCTCAGTGGCACCG	TCGGATATCTTTGCCCTTGG	55.0	50.0	57.9	57.4	646-653 bp	1.0	77.4
psaJ-rpl33_p3	AGTGGCACCGGTACTAAGTA	TCGGATATCTTTGCCCTTGG	50.0	50.0	57.8	57.4	636-643 bp	1.0	76.9
psaJ-rpl33_p4	TGGCACCGGTACTAAGTACT	TCGGATATCTTTGCCCTTGG	50.0	50.0	57.8	57.4	634-641 bp	1.0	76.9
psaJ-rpl33_p5	ATGGTTCGGATCTTTAGCGG	TCGGATATCTTTGCCCTTGG	50.0	50.0	57.8	57.4	612-619 bp	1.0	76.9