Hotspot

ndhK-ndhC

LSC · 53397-53457 · 61 bp

Back to Averrhoa

Pi 0.0656

MarkerSeek score 73.10

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 4
pi: 0.0656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AGAGAAGCGGCCATAAACTG	TGGTTCAGTCTATGCATGGC	50.0	50.0	58.0	58.0	222 bp	1.0	80.9
ndhK-ndhC_p2	AGAGAAGCGGCCATAAACTG	GTTCAGTCTATGCATGGCGA	50.0	50.0	58.0	58.1	220 bp	1.0	80.7
ndhK-ndhC_p3	AGAGAAGCGGCCATAAACTG	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	208 bp	1.0	80.6
ndhK-ndhC_p4	ACCATAGAGAAGCGGCCATA	TGGTTCAGTCTATGCATGGC	50.0	50.0	58.3	58.0	227 bp	1.0	78.8
ndhK-ndhC_p5	ACCATAGAGAAGCGGCCATA	GTTCAGTCTATGCATGGCGA	50.0	50.0	58.3	58.1	225 bp	1.0	78.7