Hotspot

trnG-GCC-trnfM-CAU

LSC · 39445-39588 · 144 bp

Back to Averrhoa

Pi 0.0347

MarkerSeek score 69.20

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 5
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	210 bp	1.0	81.1
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	211 bp	1.0	81.1
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	204 bp	1.0	80.6
trnG-GCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	205 bp	1.0	80.6
trnG-GCC-trnfM-CAU_p5	AGAAGATGCGGGTTCGATTC	GTTGCCGGTTCTTCTCTTCA	50.0	50.0	58.1	58.1	271 bp	1.0	80.0