Hotspot

psbZ-trnG-GCC

LSC · 38714-39373 · 660 bp

Back to Averrhoa

Pi 0.0556

MarkerSeek score 79.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 31
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	751-825 bp	1.0	86.6
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	GAATCGAACCCGCATCTTCT	55.0	50.0	58.0	58.1	752-826 bp	1.0	86.5
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	754-828 bp	1.0	85.0
psbZ-trnG-GCC_p4	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	755-829 bp	1.0	85.0
psbZ-trnG-GCC_p5	TCCTGATGGTTGGTCGAGTA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	747-821 bp	1.0	84.9