Hotspot

psbC-trnS-UGA

LSC · 37896-38091 · 196 bp

Back to Averrhoa

Pi 0.0737

MarkerSeek score 79.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 14
pi: 0.0737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCATTTATGGCATGCGGGAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.9	58.2	316-322 bp	1.0	82.8
psbC-trnS-UGA_p2	TCATTTATGGCATGCGGGAA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.9	58.2	366-372 bp	1.0	82.4
psbC-trnS-UGA_p3	TTTATGGCATGCGGGAAGAG	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	363-369 bp	1.0	80.4
psbC-trnS-UGA_p4	TCATTTATGGCATGCGGGAA	TTTCTTTCGGAGAGATGGCC	45.0	50.0	57.9	57.6	394-400 bp	1.0	80.3
psbC-trnS-UGA_p5	TCATTTATGGCATGCGGGAA	ATGGCTCCGGTCTTGAAAAC	45.0	50.0	57.9	58.5	365-371 bp	1.0	80.1