Hotspot

atpF-atpH

LSC · 14151-14668 · 518 bp

Back to Averrhoa

Pi 0.0509

MarkerSeek score 74.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 23
pi: 0.0509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AAAGAATCGGTTACATTTTTCA	ATTTGCAAATCCCTTTGTTT	27.3	30.0	52.6	52.3	502-563 bp	1.0	47.5
atpF-atpH_p2	AAGAATCGGTTACATTTTTCAT	ATTTGCAAATCCCTTTGTTT	27.3	30.0	52.4	52.3	501-562 bp	1.0	47.5
atpF-atpH_p3	GAAAGAATCGGTTACATTTTTCA	TTTGCAAATCCCTTTGTTTAA	30.4	28.6	53.9	52.6	502-563 bp	1.0	47.5
atpF-atpH_p4	GAAAGAATCGGTTACATTTTTCA	ATTTGCAAATCCCTTTGTTT	30.4	30.0	53.9	52.3	503-564 bp	1.0	47.4
atpF-atpH_p5	GAAAGAATCGGTTACATTTTTCAT	ATTTGCAAATCCCTTTGTTT	29.2	30.0	54.5	52.3	503-564 bp	1.0	47.4