Hotspot

trnS-GCU-trnG-UCC

LSC · 9056-9814 · 759 bp

Back to Averrhoa

Pi 0.0314

MarkerSeek score 78.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 21
pi: 0.0314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CGAAGGACCCCCTAACTATT	50.0	50.0	58.0	56.0	807-863 bp	1.0	70.1
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	CGAAGGACCCCCTAACTATT	50.0	50.0	58.0	56.0	806-862 bp	1.0	70.1
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	CGAAGGACCCCCTAACTATT	50.0	50.0	58.2	56.0	861-917 bp	1.0	68.8
trnS-GCU-trnG-UCC_p4	AGCAATCCGACGCTTTAGTC	CGAAGGACCCCCTAACTATT	50.0	50.0	58.4	56.0	809-865 bp	1.0	67.2
trnS-GCU-trnG-UCC_p5	CGACGCTTTAGTCCACTCAG	CGAAGGACCCCCTAACTATT	55.0	50.0	58.4	56.0	802-858 bp	1.0	67.1