Hotspot

trnK-UUU-rps16

LSC · 4689-5744 · 1056 bp

Back to Averrhoa

Pi 0.0254
MarkerSeek score 67.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1056
variable_sites
25
pi
0.0254
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCAAAGAAGGCGGGGATTTT 50.0 45.0 58.2 57.7 1139-1144 bp 1.0 82.0
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTCAAAGAAGGCGGGGATTT 50.0 45.0 58.2 57.7 1140-1145 bp 1.0 82.0
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TCAAAGAAGGCGGGGATTTT 50.0 45.0 58.2 57.7 1138-1143 bp 1.0 82.0
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCAAAGAAGGCGGGGATTT 50.0 45.0 58.2 57.7 1139-1144 bp 1.0 82.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTTCAAAGAAGGCGGGGATT 50.0 45.0 58.2 57.7 1140-1145 bp 1.0 82.0