Hotspot

trnH-GUG-psbA

LSC · 307-777 · 471 bp

Back to Averrhoa

Pi 0.0543
MarkerSeek score 80.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
471
variable_sites
24
pi
0.0543
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 537-547 bp 1.0 86.0
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 551-561 bp 1.0 85.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 550-560 bp 1.0 85.9
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 540-550 bp 1.0 85.9
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 552-562 bp 1.0 85.8