Hotspot

rpl16

LSC · 77235-78693 · 1459 bp

Back to Avena

Pi 0.00368
MarkerSeek score 33.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1459
variable_sites
25
pi
0.00368
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TTATAACCGCTGCGTTGTCA CTTCGCATTCTGTCCAGTGA 45.0 50.0 57.9 57.9 1973-1980 bp 1.0 80.6
rpl16_p2 TTATAACCGCTGCGTTGTCA TCTTCGCATTCTGTCCAGTG 45.0 50.0 57.9 57.9 1974-1981 bp 1.0 80.6
rpl16_p3 TCGGATCTTTCTAGGGGCAT CTTCGCATTCTGTCCAGTGA 50.0 50.0 57.9 57.9 1881-1888 bp 1.0 80.5
rpl16_p4 TCGGATCTTTCTAGGGGCAT TCTTCGCATTCTGTCCAGTG 50.0 50.0 57.9 57.9 1882-1889 bp 1.0 80.5
rpl16_p5 TTCGGATCTTTCTAGGGGCA CTTCGCATTCTGTCCAGTGA 50.0 50.0 58.1 57.9 1882-1889 bp 1.0 80.3